Yangzheng Xiaoji plays an anti-tumor role by antagonizing the action of HGF and its receptor cMET in human lung cancer cells

Hepatocyte growth factor (HGF) is a cytokine that has a profound effect on cancer cells by stimulating their migration and invasion and acting as a angiogenic factor. In cancer, the factor also plays a key role and has been linked to poor outcomes for patients. In particular, HGF is known to act in combination with EGF on cancer cells. In this study, we investigated the effects of Yangzheng Xiaoji (YZXJ), a traditional Chinese medicine reported in cancer treatment, on cancer and HGF mediated lung cancer cell migration and invasion.

method

Human cancer cells, SKMES1 and A549 were used in this study. An extract of the medicine was used. Study of cell migration using EVOS and ECIS. Assessment of cell-matrix adhesion and in vitro invasion. In vivo growth of cancer was tested using an in vivo xenograft tumor model and HGF receptors in lung tumors were activated by immunofluorescence methods.

consequence

Both cancer cells increased their migration to HGF and responded to YZXJ by reducing the rate of migration. Yangzheng Xiaoji capsules can significantly reduce HGF induced cell migration and invasion in vitro. It acts synergistically with PHA666552 and SU11274, HGF receptor inhibitors, on HGF receptor activation and cell function in cancer cells. The combination of HGF and EGF led to a greater increase in cell migration, and YZXJ as well as combinations with HGF receptors and EGF receptor inhibitors similarly inhibited cell migration. In vivo, YZXJ reduced the rate of tumor growth and enhanced the effect of PHA6665752 on tumor growth. It was further revealed that YZXJ significantly reduced the phosphorylation of HGF receptors in lung tumors.

conclusion

YZXJ plays an important role in reducing cancer migration, invasion, and tumor growth in vivo, and inhibits HGF and HGF/ EGF-induced migration and invasion effects. This effect may be attributed to inhibition of HGF receptor activation. These results suggest that YZXJ has a therapeutic role in cancer, and strategies to block HGF and EGF in combination with methods should be considered.